Antioxidant, Anti-Aging and Organ Protective Effects of Tota | DDDT – Dove Medical Press


Since the 21st century, with the global trend of aging and the improvement of living standards around the world, health problems caused by aging and aging-related diseases have become increasingly prominent.1 Therefore, how to maintain the health of elderly people and delay the occurrence of aging and aging-related diseases has become a popular research topic. Aging is an irreversible and complex degenerative physiological process that occurs in all parts of the body, leading to structural and functional deterioration of tissues and organs and increasing the risk of various chronic diseases, such as hypertension, diabetes, neurodegenerative diseases and skin melanosis.2 Several reports have shown that the free radical theory of aging, one of the most popular theories, can explain the damage to the organism caused by the aging process.3 During aerobic metabolism, reactive oxygen species (ROS) are produced in cells, including singlet oxygen (1O2), superoxide anions (•O2-), hydroxyl radicals (HO•), and hydrogen peroxide (H2O2).4,5 Once these free radicals, which contain unpaired and highly reactive electrons, are produced in excess, they directly lead to oxidative stress and proinflammatory responses, followed by redox imbalance, leading to protein and lipid peroxidation, as well as mitochondrial and DNA damage, ultimately leading to altered cell and tissue function and pathological conditions to accelerate aging.6,7 Interestingly, natural endogenous antioxidant systems exist in the human/mammalian system, including those involving SOD, CAT and GSH-Px, as well as nonenzymatic antioxidants such as ascorbic acid and carotenoids, designed to maintain a proper balance between free radicals and antioxidants.8,9 Nevertheless, the antioxidant defence system is not sufficient to completely prevent oxidative damage caused by excess free radicals, and supplementation with antioxidants can at least reduce the extent of aging and the related oxidative damage.10 Compared with natural antioxidants, synthetic antioxidants (eg, BHT and BHA) have been shown to cause endocrine disruption, reproductive disorders and even carcinogenesis in long-term use.11,12 Therefore, the search for natural, safe and effective free radical scavengers is of great importance for human health protection.

Aralia taibaiensis is a plant species of the same genus as Aralia elata (Miq.) Seem.13 It is widely distributed in the Qinba Mountains of western China and is a medicinal and edible plant. Aralia taibaiensis root bark extract has long been used in China as a folk medicine for the treatment of diabetes, and its main active ingredient is the triterpene saponin.14 It has been shown to have antioxidant, antihyperglycaemic and antihyperlipidaemic effects.14–16 Many studies have shown that triterpene saponins such as asiaticoside B, madecassoside and parkioside A can react with free radicals and prevent further development of free radical chain reactions, thus exerting antioxidant effects.17,18 Previous reports described that TSAT exhibited superior DPPH radical scavenging activity to single saponins, as well as good antioxidant capacity against lipid peroxidation in rat liver microsomes, and was shown to have superior antioxidant effects to ginseng, schisandra and other saponins.14,15 In addition, TSAT also attenuated D-gal-induced liver damage in aging rats through its antioxidant effect.19 Further structure-effect relationship study suggested that the structural differences such as the category and the sequence of the oligosaccharide chain at C-3 in such saponins play an important role in terms of diabetes mellitus related antioxidant and antiglycation activities.20

D-gal is a commonly used experimental aging inducer. Increasing amounts of evidence suggest that aging-related phenotypes such as excessive free radical formation, cognitive dysfunction, decreased immune response and organ injury can be observed under long-term D-gal stimulation.21 Behaviourally, D-gal administration leads to deficits in spatial and recognition memory in rats, as demonstrated by poor performance in terms of difficulties in finding target platforms and reduced number of times crossing target platforms in a Morris water maze experiment.22 Therefore, chronic D-gal stimulation has been used to study organismal aging and aging-related memory loss, redox imbalance and organ injury.

On the basis of previous studies on the biological activity of TSAT, there is reason to believe that this medicinal and dietary plant has considerable potential for application in the field of anti-aging. However, there is only one study on the in vitro scavenging of free radicals by this triterpenoid saponin extract, and other physiologically relevant characterizations of free radical scavenging are lacking. Furthermore, knowledge on the protective effects of TSAT against memory loss, redox imbalance and organ injury in a D-gal-induced subacute aging model is limited. Therefore, this study consolidated the antioxidant properties of TSAT in vitro by scavenging DPPH, ABTS, HO•, •O2- and inhibiting tyrosinase activity. Moreover, behavioural experiments, measurement of oxidative stress parameters and histopathological conditions of major organs in rats were evaluated to provide additional information on TSAT’s ability to ameliorate D-gal-induced subacute oxidative and aging damage, consolidating its anticipated pharmacological properties.

Materials and Methods

The root bark of Aralia taibaiensis was collected from the Qinba Mountains, Shaanxi Province, China, and was botanically identified by Dr. Jitao Wang (Shaanxi University of Chinese Medicine). A voucher specimen (SUCM, No. 20201003) was deposited in the Herbarium of Shaanxi University of Chinese Medicine. Tarasaponin IV (≥98%), araloside C (≥98%), stipuleanoside R2 (≥98%), pseudoginsenoside RT1 (≥98%), araloside A (≥98%) and chikusetsu saponin Iva (≥98%) standards were provided by Chenguang Biotechnology Co., Ltd (Baoji, China). DPPH (Sigma‐Aldrich, St. Louis, MO, USA), ABTS (Maclin Biochemical Co., Ltd, Shanghai, China), mushroom tyrosinase (Yuan Ye Biotechnology Co., Ltd, Shanghai, China), L‐3,4‐dihydroxyphenylalanine (L‐DOPA) and D‐gal (Yuan Ye Biotechnology Co., Ltd, Shanghai, China) were purchased. Superoxide anion (Item number: A052-1-1), Hydroxyl free radical (Item number: A018-1-1), BCA (Item number: A045-4-2), SOD (Item number: A001-3-2), GSH‐Px (Item number: A005-1-1), CAT (Item number: A007-1-1), MDA (Item number: A003-1-2), T-AOC (Item number: A015-2-1), AST (Item number: C010-2-1) and ALT (Item number: C009-2-1) assay kits were obtained from Nanjing JianCheng Bioengineering Institute (Nanjing, China). All chemicals and other reagents used were analytical grade.

Extraction of TSAT

Aralia taibaiensis root bark was washed with double distilled water, dried and crushed at 60 °C. Before the experiment, the powder was placed in a cool and dry place. The powder (3 kg) was extracted with 70% ethanol for three times under reflux. The solutions were combined and the supernatant was obtained by high speed centrifugation and filtration. The supernatant was purified by loading into an HPD300 macroporous resin column and then eluting with 70% ethanol. The solution which eluted with 70% ethanol was evaporated to yield a residue (356 g) after solvent removal in vacuo. The yield of TSAT was 11.87% (w/w) and was preserved in a refrigerator for further use.

HPLC Analysis of TSAT

The purified TSAT was analysed by HPLC-DAD (Agilent 1260 series liquid chromatograph, USA), which was equipped with a COSMOSIL 5C18-MS-II chromatographic column (4.6 ID×250 mm). The mobile phase consisted of acetonitrile (solvent A) and 0.1% phosphoric acid aqueous solution (V/V) (solvent B). The gradient programme was as follows: 0–10 min, 5–20% A; 10–25 min 20–28% A; 25–35 min, 28–33% A; 35–45 min, 33–38% A; 45–55 min, 38–46% A; 55–60 min, 46–60% A; 60–70 min. 60–75% A; 70–80 min, 75–45% A; 80–90 min, 45–5% A. The operating conditions of the instrument were a temperature of 30 °C and a flow rate of 0.8 mL/min. The injection volume of standards and samples was 10 μL. The detection wavelength is 203 nm. Before injection, all samples were passed through a 0.22 μm filter. After matching with the standard, the peak is determined by the retention time. The linear calibration curve of the standard was used for quantification.

Antioxidant Activity in vitro

DPPH Assay

The DPPH assay was slightly modified from a previous report.23 A mixture of anhydrous ethanol (100 μL), DPPH solution (100 μL, 0.2 mM) and sample (100 μL, 0.01–1 mg/L) was shaken vigorously and kept (25 °C, 30 min) in the dark. VC was used as a positive control. The absorbance (517 nm) was measured. The DPPH radical scavenging rate was calculated as follows:


where A0 represents the absorbance of the negative control (DPPH plus absolute ethanol); A1 represents the test system containing the sample (DPPH plus TSAT); and A2 is the absorbance of the blank system (TSAT plus absolute ethanol). The 50% inhibition concentration value (IC50) of the DPPH free radical scavenging rate was calculated, which represents the antioxidant capacity of TSAT. All operations are performed in parallel three times.

ABTS+ Assay

ABTS was determined using the method of Rafique et al with slight modifications.24 First, a series of TSAT (0.01–1 mg/mL) solutions were prepared. An ABTS solution of 7 mM and a K2S2O8 solution of 2.45 mM were prepared. After being mixed in the same volume, ABTS+ was obtained by reacting (12–16 h) in the dark. The prepared ABTS+ was diluted with double distilled water to an absorbance of 0.7 ± 0.05 at 734 nm. Then, the mixture of 180 μL diluted ABTS+ solution and 20 μL sample were reacted (25 °C, 10 min) in the dark. VC was used as the positive control. The absorbance (734 nm) was measured. The ABTS+ scavenging capacity was calculated by the following formula:


where A0 represents the absorbance of the negative control (ABTS+ plus double distilled water); A1 represents the test system containing the sample (ABTS+ plus TSAT); and A2 is the absorbance of the blank system (TSAT plus double distilled water). The IC50 value of ABTS+ scavenging rate was then calculated. All operations were performed in parallel three times.

Determination of Mushroom Tyrosinase Inhibition Activity

The tyrosinase inhibitory activity was determined according to a slightly improved version of the method previously reported by Morais et al.25 A series of 50 μL TSAT (0.01–1 mg/mL, in 50% DMSO) solutions were added to the reaction system containing 100 μL of PBS buffer (0.1 M, pH 6.8) and 50 μL tyrosinase (100 U/mL), respectively, and reacted at 25 °C for 15 min. Then, 50 μL L L-DOPA (3.5 mM) solution was added to the reaction system and incubated at 37 °C for 10 min. The absorbance (475 nm) was determined. VC was used as the positive control. The inhibition activity of TSAT was calculated by the following formula:


where A0 represents the absorbance of the negative control with 50% DMSO instead of the sample; the system contains L-DOPA and tyrosinase; A1 represents the test system containing the sample and contains L-DOPA and tyrosinase; and A2 is the blank system (sample plus L-DOPA) absorbance. The IC50 value was calculated to indicate the tyrosinase inhibitory activity of TSAT. All operations were performed in parallel three times.

Hydroxyl Radical (HO•) Scavenging Assay

The Fenton reaction is the most common chemical reaction that produces hydroxyl radicals. This experiment was carried out according to the instructions of the hydroxyl radical assay kit. Different concentrations of TSAT (0.01–1 mg/mL) were mixed with the reagents sequentially and left at room temperature for 20 min. Absorbance (550 nm) was measured, and VC was used as a positive control. The scavenging rate (%) was calculated as follows:


where A0 is the absorbance of the negative control system without TSAT, A1 is the absorbance of the reaction system containing TSAT, and A2 is the absorbance of the reaction system containing double distilled water instead of H2O2. The IC50 value was then calculated. All operations were performed in parallel three times.

Superoxide Radical (•O2-) Scavenging Assay

The inhibition of superoxide anion radical kits has biological significance by simulating the reaction system of xanthine and xanthine oxidase to generate superoxide anion radicals. In this experiment, the reagents were prepared according to the kit instructions, different concentrations of TSAT solution (0.01–1 mg/mL) were added, and a water bath at 37 °C for 40 min. VC was used as a positive control. The scavenging rate (%) was calculated as follows:


where A0 represents the absorbance of the negative control with deionized water instead of the sample; A1 represents the test system containing the sample; and A2 is the absorbance of the blank system. The IC50 value was then calculated. All operations were performed in parallel three times.

Antioxidant Activity in vivo

Animals and Treatment

Male Sprague-Dawley rats (200–230 g) were supplied by the Chengdu Dossy Experimental Animals co., Ltd (ChengDu, China, certificate no. SCXK2020-030). All animal experiments were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Ethics Committee of Shaanxi University of Chinese Medicine. These rats were raised in a climate control room (24–25 °C) according to a 12 h light/12 h dark schedule and had free access to food and water. All rats were allowed to adapt for a week before starting the experiment. The rats were randomly divided into 6 groups (n=8 in each group): normal group; model group; VC treatment group (100 mg/kg); and TSAT high (TSAT-H)-, medium (TSAT-M)- and low (TSAT-L)-dose treatment groups (200 mg/kg, 100 mg/kg, 50 mg/kg). All five groups were injected intraperitoneally with D-gal (200 mg/kg) except for the normal group, which was treated with 0.9% NaCl (10%, w/v). The rats in the treatment group were given the drug by gavage once daily, and the dose was continuously adjusted according to body weight for 8 weeks.

After the last administration, the rats fasted for one day. Blood samples were collected immediately after anaesthesia in all rats, and serum was collected by centrifugation (3500 rpm, 15 min, 25 °C). The thymus, heart, liver, spleen, lung, kidney, and testicular tissues were separated quickly, rinsed with ice 0.9% NaCl solution, and weighed accurately for the following analysis.

Morris Water Maze (MWM) Test

The Morris water maze (MWM) experiment was performed according to a previous description with slight modifications.26 Briefly, the rats were trained for five running days (6 trials/per day) to find the escaped platform, which was hidden 1 cm in water (water temperature, 25±1 °C) using a fixed array of cues outside the swimming pool (diameter 160 cm, height 60 cm). The swimming pool was divided into four equal quadrants. The arrowhead cues around the pool were kept in the same location throughout the whole training and testing time. After a five-day training, each mouse was allowed to swim for 120 s. After finding the platform, rats were allowed to stay there for 15 s. Beyond that, the rat that failed to locate the platform within the stipulated period (120 s) was placed on it for 15 s. The escape latencies of these rats were recorded as 120 s. Later, the platform was removed, and the rats were placed in the quadrant, which was contrary to the target quadrant, and were allowed to swim freely for 120 s. The numbers of platform quadrant crossings were recorded in the target quadrant. The data were analysed by an automated tracking system.

Biochemical Analysis

The brain, lung, liver, spleen, heart and kidney tissues were homogenized quickly in ice-cold 0.9% NaCl solution (10%, w/v). After centrifugation (3500 rpm, 15 min, 25 °C), the supernatant was collected for biochemical analysis. Using bovine serum albumin as the standard, the tissue protein concentration was determined. The levels of SOD, GSH-Px, CAT, MDA, and T-AOC in rat serum and brain, heart, lung, spleen and kidney homogenates were determined according to the standard procedures of the kit, and the liver ALT and AST levels were determined according to the kit instructions.

Histopathological Analysis

The brain, heart, lung, kidney, liver and spleen tissues were quickly removed and fixed in 4% paraformaldehyde. 24 h later, paraffin embedding was performed. Sections were sectioned longitudinally to 4 µm thickness, hydrated and stained with haematoxylin-eosin (H&E). Histomorphology was observed microscopically.

Statistical Analysis

The results of the behaviour tests, organ index and enzyme activities are expressed as the means ± SD. The data were analysed by one-way analysis of variance (ANOVA) followed by Tukey’s test for post hoc analysis. For novel object recognition, statistical evaluation was performed by a paired sample test. Statistical significance was set at p <0 05.


Analysis of TSAT Composition

As shown in Figure 1, HPLC analysis revealed that TSAT contained six types of saponins, including tarasaponin IV, araloside C, stipuleanoside R2, pseudoginsenoside RT1, araloside A and chikusetsu saponin Iva. Table 1 shows the content of six saponins in TSAT.

Table 1 Contents of TSAT Saponins

Figure 1 Saponins constituents of TSAT. (A) Standard chromatograms; (B) Saponins of TSAT chromatograms. 1: Tarasaponin IV; 2: Araloside C; 3: Stipuleanoside R2; 4: Pseudoginsenoside RT1; 5: Araloside A; 6: Chikusetsu saponin IVa.

Antioxidant Capacities in vitro

As shown in Figure 2A, TSAT provided single electrons for pairing with DPPH radicals, and as the concentration of TSAT increased (0.01–1 mg/mL), the colour of the reaction system gradually became lighter, obeying a significant dose-dependent relationship. The reaction system was almost colourless when TSAT concentrations reached 1 mg/mL, reaching a scavenging rate of 90.5 ± 1.4%, close to that of VC. In addition, the IC50 values of DPPH radical scavenging by TSAT and VC were 0.27 mg/mL and 5.19 μg/mL, respectively. The results indicate that TSAT has the ability to scavenge DPPH free radicals.

Figure 2 Antioxidant activities of TSAT in vitro. (A) DPPH radicals, (B) ABTS radicals, (C) Tyrosinase, (D) Hydroxyl radicals and (E) Superoxide anion radicals.

Abbreviation: VC, Vitamin C.

The scavenging effect of TSAT on ABTS free radicals is shown in Figure 2B. When the concentration increased from 0.01 to 1 mg/mL, the scavenging rate increased from 1.56 ± 3.46% to 56.01 ± 3.40%. It was found that the scavenging effect of TSAT on ABTS free radicals was dose-dependent. The IC50 value was 0.78 mg/mL. This shows that increasing the concentration of TSAT can significantly improve the scavenging ability of ABTS free radicals. Under the same conditions, the IC50 value of VC scavenging ABTS free radicals was 15.48 μg/mL.

The tyrosinase inhibitory activity of TSAT was evaluated with L-DOPA as a substrate in vitro. As shown in Figure 2C, the inhibition rate of tyrosinase activity by TSAT obeyed a dose dependence at concentrations of 0.01–1 mg/mL. The inhibition rate of tyrosinase activity by TSAT was 48.55 ± 2.62%, and the IC50 value was 1.18 mg/mL at 1 mg/mL. TSAT was proven to be a potential tyrosinase activity inhibitor in the present study. In this study, it was proven to be a potential tyrosinase activity inhibitor for the first time; thus, it may have a potential whitening effect.

Figure 2D shows that the clearance of HO• by TSAT was only 3.04 ± 1.93% at a concentration of 0.01 mg/mL and reached 56.86 ± 1.31% at 1 mg/mL with increasing TSAT concentration. The IC50 value was 0.528 mg/mL. In conclusion, TSAT has a good scavenging capacity for HO•. It is speculated that TSAT may act as a metal ion chelating agent to block the formation of HO•.

In this study, the scavenging rate of •O2- by TSAT was 39.94 ± 0.49% at a concentration of 1 mg/mL, with an IC50 value of 2.43 mg/mL. In addition, Figure 2E shows that the scavenging rate in •O2- quenching correlated with the concentration of TSAT.

Anti-Aging Activities Assaying

Daily Behaviour Observations and Organ Indices

During the eight weeks of our experiment, the daily appearance and behavioural activities of the rats were observed and recorded weekly. The rats in the normal group were not only in good mental condition but were also lively, active and agile and had a healthy diet. As shown in Figure 3A, after the 5th week of D-gal injection, the rats in the model group showed a decline in body weight accompanied by obvious signs of senescence, including slow movement, gradually reduced food intake, depression and dry fur, indicating that the senescence model triggered by intraperitoneal injection of D-gal had been successfully established. The above senescence features were regressed to different degrees in both the VC group and the treated rats compared with the model group. In particular, in the high-dose TSAT-treated group, the abovementioned aging characteristics of the rats were significantly reduced compared with those of the model group. Aging causes degenerative atrophy of organs, leading to a decrease in organ indices.27,28 In this study, thymus, liver, spleen, kidney and heart organ indices were highest in normal control rats and lowest in the model group (p<0.05) (Figure 3), indicating that D-gal caused organ atrophy in rats. Notably, the TSAT-induced increase in organ indices may reflect the prevention of atrophy of body tissues.

Figure 3 (A) Body weight, (B) thymus coefficient, (C) liver coefficient, (D) spleen coefficient, (E) kidney coefficient and (F) heart coefficient. *p<0.05 and ***p<0.0001 vs. normal group; #p<0.05 and ###p<0.0001 vs. model group. The data are presented as the means ± SD (n=8).

Morris Water Maze (MWM) Test

We used the MWM test to assess the effects of TSAT on spatial learning and memory abilities in a D-gal-induced mouse model. As shown in Figure 4A and B, rats in the D-gal group suffered a significant impairment in spatial learning ability during the 5-day location navigation training, as evidenced by a longer escape latency compared to control rats, whereas rats treated with TSAT had a significantly shorter escape latency, which was closer to the normal level. To assess spatial memory more directly, rats were subjected to another test in which the target platform was removed the day after navigation training. As shown in Figure 4C, the rats given D-gal crossed the location less often than the control group. However, compared to those in the model group, the rats in the treatment group had less time to escape the latency and crossed the platform more often, with the higher dose group being more significant (p<0.05). These results suggest that the aging model impaired spatial learning and memory, while oral administration of TSAT restored age-related cognitive deficits induced by D-gal exposure.

Figure 4 Morris water maze test. (A) Representative pictures of swimming traces on the 1st day and 5th day in the D-gal model; (B) TSAT treatment reduced escape latency time in the D-gal model. (C) TSAT treatment increased across platform times in the D-gal model. ***p<0.0001 vs. normal group; #p<0.05 vs. model group. The data are presented as the means ± SD (n=8).

Oxidation-Related Index Assays

SOD levels in serum, brain, heart, lung, spleen and kidney were significantly lower in the untreated model group than in the normal group (p<0.01) (Figure 5), indicating that the organism was damaged by oxidative stress to different degrees. However, the SOD levels in the aging rats administered different doses of TSAT were significantly higher than those in the model group (p<0.05). The results indicated that TSAT could reverse the decrease in SOD content in aging tissues and serum, with the best effect in the high-dose group.

Figure 5 Effect of TSAT on SOD levels in (A) serum, (B) brain, (C) heart, (D) lung, (E) spleen and (F) kidney. ***p<0.0001 vs normal group; #p<0.05, ##p<0.01 and ###p<0.0001 vs model group. All the data are expressed as the means ± SD of 6–8 rats in each group.

Compared with the MDA value of normal rats, the MDA levels in the serum, brain, heart, lung, spleen and kidney of the untreated model group were significantly increased (p<0.01) (Figure 6), indicating that organs suffer from oxidative stress injury. In contrast, TSAT treatment downregulated MDA accumulation (p<0.05). The results indicate that the potential anti-aging properties of TSAT may be related to its reduction in MDA content.

Figure 6 Effect of TSAT on MDA levels in (A) Serum, (B) Brain, (C) Heart, (D) Lung, (E) Spleen and (F) Kidney. ***p<0.0001 vs normal group; #p<0.05, ##p<0.01 and ###p<0.0001 vs model group. All the data are expressed as the means ± SD of 6–8 rats in each group.

The levels of CAT were significantly lower (p<0.01) in the serum, brain, heart, lung, spleen and kidney of rats in the untreated model group than in the normal group (Figure 7), indicating that the accumulation of H2O2 in the organism led to structural cell damage. However, CAT levels improved after treatment in both the VC and TSAT administration groups (p<0.05), especially in the serum and organ CAT levels of rats in the high-dose TSAT group, which were significantly higher than those in the model group.

Figure 7 Effect of TSAT on CAT levels in (A) Serum, (B) Brain, (C) Heart, (D) Lung, (E) Spleen and (F) Kidney. **p<0.001, ***p<0.0001 vs normal group; #p<0.05, ##p<0.01 and ###p<0.0001 vs model group. All the data are expressed as the means ± SD of 6–8 rats in each group.

GSH-Px was significantly lower (p<0.01) in the serum, brain, heart, lung, spleen and kidney of untreated aging rats (Figure 8) than in the normal group of rats. In contrast, GSH-Px activity was significantly increased after TSAT administration, with a significant difference compared to that in the model group (p<0.05), possibly acting by blocking free radical damage to cell membrane lipids.

Figure 8 Effect of TSAT on GSH-PX levels in (A) Serum, (B) Brain, (C) Heart, (D) Lung, (E) Spleen and (F) Kidney. ***p<0.0001 vs normal group; #p<0.05, ##p<0.01 and ###p<0.0001 vs model group. All the data are expressed as the means ± SD of 6–8 rats in each group.

As shown in Figure 9, serum, brain, heart, lung, spleen and kidney T-AOC levels were significantly lower in the untreated aging group than in the normal group (p<0.01). However, TSAT treatment significantly increased T-AOC levels (p<0.05). This may be due to the long-term consumption of antioxidant enzymes caused by high levels of oxidative stress, which was reversed by oral TSAT. This result strongly indicates that TSAT has antioxidant potential and can increase antioxidant capacity, thus reducing the damage caused by aging.

Figure 9 Effect of TSAT on T-AOC levels in (A) Serum, (B) Brain, (C) Heart, (D) Lung, (E) Spleen and (F) Kidney. ***p<0.0001 vs normal group; #p<0.05, ##p<0.01 and ###p<0.0001 vs model group. All the data are expressed as the means ± SD of 6–8 rats in each group.

ALT and AST are effective markers for evaluating liver function injury.29 As shown in Figure 10, compared with those in the normal group of rats, the levels of ALT and AST in the untreated model group were significantly increased (p<0.01), indicating that the liver was damaged by D-gal stimulation. In contrast, after administration of TSAT, the levels of ALT and AST in the livers of aging rats were significantly reduced (p<0.05). The results show that TAST has a protective effect on the liver.

Figure 10 Effect of TSAT on (A) ALT and (B) AST levels in liver. ***p<0.0001 vs normal group; #p<0.05, ##p<0.01 and ###p<0.0001 vs model group. All the data are expressed as the means ± SD of 6–8 rats in each group.

Histopathological Observations

In this study, histopathological observations were performed on the brain, heart, lung, kidney, liver and spleen to confirm the evidence from the biochemical analysis. Figure 11 shows the effect of TSAT on the histopathology of D-gal-induced aging rats. It is obvious that organ injury is mainly manifested by the degradation of cells and nuclei. In addition, senescent rats exhibited other pathological patterns in these organs, such as partial edema, fragmented arrangement of nerve cells and atrophy of nuclei in brain tissue (Figure 11A), broken, loosely arranged and increased intercellular fibres in the myocardium (Figure 11B), alveolar haemorrhage, thickened alveolar walls and inflammatory cell infiltration in the alveolar space and around blood vessels (Figure 11C), glomerular atrophy and possible proximal tubules of the kidney balloon dilatation lumen (Figure 11D), binucleation of hepatocytes, hepatic cord disorder and swelling of hepatocytes (Figure 11E), and old haematopoiesis in the spleen with increased fibrous septa and fused lymphoid follicles (Figure 11F). Interestingly, TSAT treatment significantly attenuated the adverse organ lesions caused by D-gal, with the best results seen in the high-dose group. Moreover, the vitamin C-treated control rats had a similar recovery effect. The results suggest that TSAT has potential protective effects on the brain, heart, lung, kidney, liver and spleen against acute D-gal-induced injury.

Figure 11 Effects of TSAT on organ injury in the (A) Brain, (B) Heart, (C) Lung, (D) Kidney, (E) Liver and (F) Spleen in D-gal-induced aging rats (n=3).

Abbreviations: VC, rat treated with vitamin C (100 mg/kg); TSAT-H, rat treated with high concentration of TSAT (200 mg/kg); TSAT-M, rat treated with medium concentration of TSAT (100 mg/kg); TSAT-L, rat treated with low concentration of TSAT (50 mg/kg) (Haematoxylin/eosin staining, brain magnified at 400X, all other organs magnified at 200X).


In recent years, TSAT has been shown to have high antioxidant activity and contribute to the prevention of ROS-induced oxidative damage in the body. Based on the free radical theory of aging, long-term injection of D-gal causes oxidative stress by stimulating excessive production of ROS, leading to metabolic disorders and inducing oxidative damage to organs in vivo associated with aging.30,31 Therefore, D-gal-induced aging models have been widely used in anti-aging and organ injury studies, for which antioxidant supplementation may be a potential therapeutic strategy for D-gal-induced oxidative and aging damage. In this study, the antioxidant activity of TSAT was consolidated by scavenging DPPH, ABTS, hydroxyl radicals and superoxide anion radicals and inhibiting tyrosinase activity in vitro. Furthermore, behavioural evaluation, organ indices, biological indicators of oxidative stress and analysis of pathological changes revealed that D-gal-induced aging rats had reduced major organ indices and reduced learning memory capacity with varying degrees of oxidative damage, but this effect was inhibited by TSAT intervention.

HPLC was used to analyse TSAT in this study, and the results showed that TSAT contained six triterpene saponins, including araloside C, Chikusetsu saponin IVa, araloside A, pseudoinsenoside RT1, tarrasaponin IV and stipuleanoside R2. Araloside C and Chikusetsu saponin IVa have been shown to have antioxidant properties, reduce the accumulation of ROS in cells, inhibit apoptosis and reduce Ca2+ overload.32,33 It has been reported that mitochondrial dysfunction generates excess reactive oxygen species and contributes to aging-related diseases.34 Araloside C reduces mitochondrial ROS levels by increasing cell viability and improving disruption of mitochondrial membrane potential.35 However, whether other saponins have similar effects need to be further investigated. These results suggest that the anti-aging effect of TSAT is related to the scavenging effect on ROS associated with its active saponins.

Increasing evidence has shown that ROS such as superoxide anion, hydrogen peroxide and hydroxyl free radicals can attack life macromolecules and cell walls, thus inducing oxidative stress, causing various oxidative damage and pathological changes, and accelerating aging.10,36,37 The present work evaluates the antioxidant activity of TSAT by measuring the scavenging of DPPH radicals, ABTS radicals, tyrosinase, hydroxyl radicals and superoxide anions in vitro. Among them, DPPH is one of the stable and mature organic nitrogen free radicals and has the maximum absorption at 517 nm. After adding antioxidants, electrons or hydrogen atoms are transferred to DPPH to form stable DPPH-H compounds, which reduces its absorption at 517 nm.38 In ABTS analysis, ABTS was converted to ABTS+ by adding K2S2O8. In the presence of antioxidants, active ABTS+ was converted into a colourless natural form. Tyrosinase plays a key role in melanin formation and browning. Under ultraviolet radiation stimulation, the excessive production and accumulation of melanin in the skin may lead to pigmentation disorders and skin aging, and the down-regulation of tyrosinase is considered to be the reason for the decrease in melanin activity. Tyrosinase has also been reported to be associated with neurodegenerative diseases. When produced in excess, tyrosinase is able to increase intracellular dopamine production, followed by the induction of melanin formation, causing cell death.39,40 Interestingly, some studies have proved that saponins could be potential natural tyrosinase inhibitors. For example, saponins from Xanthoceras Sorbifolia nutshell were reported to inhibit 52.0% of tyrosinase activity at a concentration of 0.96 mg/mL.41 Furthermore, some transition metals including Fe2+, Cu2+ and Co2+ can trigger the free radical reactions in magnifying the cellular damages. Among these transition metals, Fe2+ is known as the most powerful prooxidant on stimulating the lipid peroxidation by generating hydroxyl radicals through Fenton reaction.42 •O2- is a product of biological and photochemical stimuli. It is highly toxic when combined with HO•, causing lipid peroxidation and damage to the normal structure of cells.43 Hence, the antioxidant and anti-aging properties of TSAT may be due to its free radical scavenging, tyrosinase inhibition and metal ion chelating. Additionally, there has report that the scavenging properties of TSAT were mainly associated with the structural differences such as the category and the sequence of the oligosaccharide chain at C-3 position in such saponins.20

Learning and memory deficits are considered to be clinical manifestations of neurodegenerative diseases associated with aging.26 Increasing evidence suggests that oxidative stress in the brain may play a key role in the pathological process of cognitive decline.44 This was confirmed in the MWM test study, where our results showed that escape latencies were longer in a D-gal-induced aging rat model, suggesting that D-gal impaired spatial learning in rats. Furthermore, the results across plateau counts also demonstrate that TSAT treatment improves spatial memory impairment in the D-gal-induced model. Moreover, cellular damage to hippocampal neurons has been shown to contribute to cognitive decline in AD, and we used H&E to evaluate the effects of TSAT on hippocampal neurons.45 Our results suggest that oral administration of TSAT has a neuroprotective effect, improving learning memory capacity and attenuating neuronal damage. This is consistent with a previous result that Aralia taibaiensis total saponin attenuates I/R-induced mitochondrial dysfunction and oxidative stress, thereby protecting brain cells from damage.46

Organ indices are indicators to assess the health status of animals.47 With aging, the body’s immune system undergoes degenerative changes that not only reduce the sensitivity of non-specific immunity but also present a general imbalance in immune function, which ultimately leads to the development of diseases. Therefore, body immunity is one of the main tools in anti-aging research. The thymus and spleen are two important immune organs in the body, and their organ indices can initially reflect the strength of the nonspecific immune system.48,49 Compared with the normal group, the organ indices of the thymus and spleen in the model group of rats were reduced. However, there was an overall increase in organ indices in the drug group, but the variability was not significant, which may be related to the individual variability of the rats. The liver is also considered to be the immune organ of the animal, and alterations in the liver index can affect the body’s immune function.50 The results showed that TSAT significantly increased the D-gal-induced decrease in the organ index, which is consistent with its traditional hepatoprotective effect. These results suggest that D-gal successfully induces oxidative aging in rats, leading to a reduction in the organ index and that TSAT produces anti-aging effects by regulating immune organs.

It is well established that antioxidant enzyme activity decreases during aging. In the present study, SOD activity was significantly reduced during D-gal-induced aging. As the most important enzyme in living organisms, SOD protects the structural integrity of cells by blocking damage to cells by •O2-. In a state of oxidative stress, the inward flow of intracellular Ca2+ leads to the irreversible conversion of xanthine dehydrogenase to xanthine oxidase, which in turn catalyses the oxidation of hypoxanthine, generating large amounts of •O2- that damage cellular structure and accelerate aging.51 Free radical overload leads to an overconsumption of SOD and reduced activity. CAT is another important antioxidant enzyme that maintains H2O2 homeostasis in the body, rapidly breaking down H2O2 into H2O and O2 and preventing abnormal accumulation of H2O2 in cells. The main role of GSH-Px is to interrupt the chain reaction of lipid peroxidation and catalyse the conversion of lipid peroxides into nontoxic hydroxyl compounds.28 In this study, we found that CAT and GSH-Px levels were significantly lower in the serum and organs of aged rats than in those of normal rats. This has been reported that this may be related to increased utilization of CAT and GSH-Px by the body’s antioxidant system.10 MDA is a product of free radical chain reaction-induced lipid peroxidation and has been used as a marker to assess oxidative damage. In the current study, free radical action on cell membranes leading to membrane lipid damage may be responsible for the significant increase in MDA levels in the serum and organs of aging rats.10,51 The results of this study are consistent with earlier reports in the literature. We found that when TSAT was administered to aging rats, the levels of SOD, CAT and GSH-Px were significantly increased, and the levels of MDA were significantly decreased. This may be due to the active components of TSAT after metabolism in vivo, which restores the activity of these enzymes by providing single electrons to pair with free radicals, thereby scavenging them. This restoration contributes to cellular integrity, reduces oxidative stress and mitigates lipid peroxidation. These results strongly confirm the ability of TSAT to delay aging by scavenging free radicals, which was consistent with the results of in vitro experiments. Compared to single antioxidants, T-AOC can reflect the collective effect of enzymatic or nonenzymatic systems involved in antioxidant defence in biological fluids and is a comprehensive measure to evaluate the total antioxidant capacity of the body.52 Thus, higher levels of T-AOC may indicate an increased protective response of individuals and decreased susceptibility to oxidative damage.53 In this study, the level of T-AOC decreased in rats exposed to D-gal for a long time, which may be due to the long-term consumption of antioxidant enzymes caused by high levels of oxidative stress, which was reversed by oral TSAT. The liver is the most vigorous metabolic organ in the body and an important detoxification organ. It plays an important role in regulating the health of the body.54 ALT is mainly found in the hepatocyte cytoplasm, while AST is mainly found in the mitochondria of hepatocytes. When liver cells are damaged, serum ALT levels increase, and when liver cells die, serum AST levels increase significantly.55 Therefore, serum ALT and AST levels can reflect the extent of liver injury. The results showed that ALT and AST levels were elevated in liver tissue after D-gal injury, but TSAT intervention inhibited the elevation of these transaminases. These results strongly suggest that TSAT attenuates oxidative damage to organs during aging to some extent by inhibiting oxidative stress. These can be demonstrated in histological observations.


In this study, we consolidated the antioxidant, anti-aging and organ protective effects of TSAT in vitro and in vivo. TSAT is a class of natural saponins with good scavenging effects on DPPH, ABTS, hydroxyl radicals and superoxide anion radicals and inhibits tyrosinase activity. In addition, TSAT exerted anti-aging effects in D-gal-induced aging rats by improving learning and memory capacity, preventing immune organ atrophy, increasing the activity of key antioxidant enzymes in the serum, brain, heart, lung, spleen and kidney, attenuating lipid peroxidation and inhibiting transaminase levels in liver tissues, thereby reducing oxidative and aging damage and exerting anti-aging effects. However, although our study is important in consolidating the antioxidant, anti-aging and organ protective effects of TSAT, its molecular regulation on key antioxidant enzymes needs to be further investigated. Furthermore, due to the use of total saponins in this study, it should be further explored which specific components of TSAT contribute to these effects. Further investigation of these effects will provide supportive preclinical evidence for the potential clinical application of TSAT as a product for the treatment of age-related diseases.


This study was supported by the National Natural Science Foundation of China (No. 81703925) and the disciplinary innovation team construction project of Shaanxi University of Traditional Chinese Medicine (2019-YL11).


The authors report no conflicts of interest in this work.


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